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细胞核生物力学研究进展

刘中乾 齐颖新

刘中乾, 齐颖新. 细胞核生物力学研究进展. 力学进展, 待出版 doi: 10.6052/1000-0992-23-053
引用本文: 刘中乾, 齐颖新. 细胞核生物力学研究进展. 力学进展, 待出版 doi: 10.6052/1000-0992-23-053
Liu Z Q, Qi Y X. The progress in nuclear biomechanics. Advances in Mechanics, in press doi: 10.6052/1000-0992-23-053
Citation: Liu Z Q, Qi Y X. The progress in nuclear biomechanics. Advances in Mechanics, in press doi: 10.6052/1000-0992-23-053

细胞核生物力学研究进展

doi: 10.6052/1000-0992-23-053
基金项目: 国家自然科学基金项目 (Nos.12032003, 12102261) 资助
详细信息
    作者简介:

    齐颖新, 医学博士, 上海交通大学生命科学技术学院特聘教授. 国家杰出青年基金和国家优秀青年基金获得者, 兼任中国力学学会理事、生物力学专业委员会副主任委员, 中国生物医学工程学会常务理事, 中国生物化学与分子生物学学会理事. 主要从事心血管疾病发生、发展中力学生物学机制的相关研究, 为揭示血管稳态维持的分子机制和血管重建的发病机理提供力学生物学依据

    通讯作者:

    qiyx@sjtu.edu.cn

The progress in nuclear biomechanics

More Information
  • 摘要: 细胞核是细胞内最大、刚度最高的细胞器, 也是遗传信息储存、复制和转录的中心场所, 在生命活动调节方面发挥关键作用. 研究表明, 生物力学因素在细胞核结构和功能的动态变化调控中至关重要. 核骨架蛋白和核孔复合体作为细胞核典型的力学响应组分, 在维持细胞核形态结构的同时将细胞骨架传递而来的力呈递至染色质, 进而影响染色质构象、基因表达等一系列细胞核相关活动, 调控细胞功能. 细胞核及其组分对应力信号的感知和转导已成为生物力学研究的前沿热点之一. 为更深入地认识生理和病理状态下细胞核的力学特性, 揭示其在细胞命运决定中的作用和机制, 本文总结了细胞核生物力学相关的研究进展, 重点介绍了细胞核骨架、核孔复合体和染色质的物理结构、力学响应过程、各组分的相互作用以及用于细胞核生物力学研究的技术进展, 最后总结了细胞核与早衰综合症、神经退行性疾病和心血管疾病的关系, 并对细胞核生物力学未来发展进行了展望.

     

  • 图  1  机械信号转导过程中的细胞核组分

    图  2  参与细胞机械信号转导的细胞骨架及细胞核骨架蛋白

    图  3  NPC的基本结构和功能

    图  4  染色质组装及其对机械信号的响应

    图  5  细胞生物力学研究中的力学加载方式进展

    表  1  不同骨架蛋白之间的力学特性比较

    蛋白名称 弹性模量 (dyn/cm2) 应变硬化 (%) 韧性 (%) 工程应变 (%) 相角δ (°)
    F-actin ~ 10
    (Xu et al. 2000)
    20
    (Janmey et al. 1991)
    10
    (Xu et al. 2000)
    - 30
    (Xu et al. 2000)
    Keratin 5-7
    (Yamada et al. 2003)
    100
    (Yamada et al. 2003)
    100-200
    (Yamada et al. 2003)
    45
    (Guthold et al. 2007)
    4-5
    (Yamada et al. 2003)
    Vimentin 4
    (Panorchan et al. 2004)
    - 12
    (Panorchan et al. 2004)
    205
    (Qin et al. 2009)
    10
    (Panorchan et al. 2004)
    Lamin 1
    (Panorchan et al. 2004)
    200
    (Panorchan et al. 2004)
    200
    (Panorchan et al. 2004)
    250
    (Sapra et al. 2020)
    10
    (Panorchan et al. 2004)
    下载: 导出CSV

    表  2  使用不同技术测量不同类型细胞核刚度的实例

    细胞类型 刚度 (kPa) 检测技术 参考文献
    小鼠胚胎成纤维细胞 0.21 ± 0.01 微吸管 (Rowat et al. 2006)
    人宫颈癌细胞
    (Hela)
    0.41 ± 0.08 微吸管 (Rowat et al. 2005)
    牛胸主动脉内皮细胞 0.42 ± 0.12 微吸管 (Deguchi et al. 2005)
    大鼠肺上皮细胞 3.10 ± 1.50 AFM (Azeloglu et al. 2008)
    大鼠肺成纤维细胞 3.30 ± 0.80 AFM (Azeloglu et al. 2008)
    人瓣膜间质细胞 9.29 ± 1.80 AFM (Liu H et al. 2014)
    人脐静脉内皮细胞 7.22 ± 0.46 AFM (Mathur et al. 2000)
    人宫颈癌细胞
    (Hela)
    6.70 ± 2.20 AFM (Nagayama et al. 2015)
    人血管平滑肌细胞 23.20 ± 14.30 AFM (Nagayama et al. 2015)
    人巩膜静脉窦内皮细胞 1.19 ± 0.19 AFM (Vargas-Pinto et al. 2013)
    人髓系白血病细胞 0.13 ± 0.06 光镊 (Zhou Z L et al. 2014)
    人肌肉上皮细胞 0.02 ± 0.01 光镊 (Coceano et al. 2016)
    人乳腺癌细胞
    (MCF-7)
    0.03 ± 0.02 光镊 (Coceano et al. 2016)
    人乳腺癌细胞
    (MDA-MB-231)
    0.02 ± 0.01 光镊 (Coceano et al. 2016)
    下载: 导出CSV
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