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原子力显微镜力谱技术及其在微观生物力学领域的应用

葛林

葛林. 原子力显微镜力谱技术及其在微观生物力学领域的应用[J]. 力学进展, 2018, 48(1): 1811. doi: 10.6052/1000-0992-17-011
引用本文: 葛林. 原子力显微镜力谱技术及其在微观生物力学领域的应用[J]. 力学进展, 2018, 48(1): 1811. doi: 10.6052/1000-0992-17-011
GE Lin. AFM force spectroscopy and its applications on micro biomechanics[J]. Advances in Mechanics, 2018, 48(1): 1811. doi: 10.6052/1000-0992-17-011
Citation: GE Lin. AFM force spectroscopy and its applications on micro biomechanics[J]. Advances in Mechanics, 2018, 48(1): 1811. doi: 10.6052/1000-0992-17-011

原子力显微镜力谱技术及其在微观生物力学领域的应用——

doi: 10.6052/1000-0992-17-011
详细信息
    通讯作者:

    葛林

  • 中图分类号: TH742, Q937;

AFM force spectroscopy and its applications on micro biomechanics

More Information
    Corresponding author: GE Lin
  • 摘要: 基于原子力显微镜技术的力谱技术是一种高灵敏度的力学检测方法.它能够以前所未有的精度, 在微观生物力学领域表征组织、细胞、生物膜、蛋白质、核酸、功能材料等目标对象, 探索其包括形貌、化学信息、导电性、静电力以及生物学特性在内的等信息, 并且能够对其进行分子级别精度的三维操纵. 从而对分子结构与构象变化, 分子间的相互作用以及反应历程实现单分子水平的实时--原位观测, 提供了其他测试方法不能完成的实验设计之可能性.本文首先介绍了原子力显微镜及其力谱技术的原理, 以及影响测量结果的各个参数的物理意义; 其次按照单个目标对象与配对目标对象的区分方式, 详细介绍了力谱技术在微观生物力学各个尺度上的研究进展; 之后介绍了力谱技术结合成像模式下的发展和应用; 最后对设备的改进和本研究领域发展方向进行了展望.

     

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